16S Ribosomal RNA Identification of Prevotella nigrescens from a Case of Cellulitis
نویسندگان
چکیده
John Jeongseok Yang, M.D., Tae Yoon Kwon, M.D., Mi Jeong Seo, M.T., You Sun Nam, M.S., Chung Soo Han, M.D., and Hee-Joo Lee, M.D. Department of Medicine, Graduate School, Kyung Hee University; Departments of Laboratory Medicine and Orthopedic Surgery, School of Medicine, Kyung Hee University, Kyung Hee University Hospital, Seoul; Department of Biomedical Science, Graduate School, Kyung Hee University, Seoul, Korea
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Direct quantitative differentiation between Prevotella intermedia and Prevotella nigrescens in clinical specimens.
This paper describes a quantitative fluorescent in situ hybridization (FISH) assay for the differential identification of Prevotella intermedia and Prevotella nigrescens in clinical samples, and compares its performance with less discriminatory culture and quantitative immunofluorescence (IF) assays. Fluorescence-labelled oligonucleotide probes directed to specific 16S rRNA sequences of P. inte...
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